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Role of sphingosine kinase in Ca 2+ signalling by epidermal growth factor receptor
Author(s) -
Meyer zu Heringdorf Dagmar,
Lass Holger,
Kuchar Igor,
Alemany Regina,
Guo Yuanjian,
Schmidt Martina,
Jakobs Karl H
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01463-5
Subject(s) - sphingosine , epidermal growth factor , sphingosine kinase , phospholipase c , sphingosine 1 phosphate , microbiology and biotechnology , intracellular , receptor , stimulation , hek 293 cells , biology , second messenger system , kinase , signal transduction , chemistry , biochemistry , endocrinology
Contribution of sphingosine kinase (SPK)‐catalyzed production of sphingosine‐1‐phosphate (SPP), in comparison to phospholipase C (PLC), to Ca 2+ signalling by epidermal growth factor (EGF) was studied in two HEK‐293 cell clones (HEK2 and HEK3), expressing functional EGF receptors and exhibiting release of stored Ca 2+ by intracellular SPP. In HEK3 cells, EGF increased [Ca 2+ ] i and stimulated both, SPK and PLC. [Ca 2+ ] i increase, but not PLC stimulation, was strongly reduced by SPK inhibition. In HEK2 cells, EGF similarly stimulated PLC, but did not increase [Ca 2+ ] i or stimulate SPK, suggesting that intracellular SPP production plays a major role for Ca 2+ signalling by EGF in HEK‐293 cells.