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Human napsin A: expression, immunochemical detection, and tissue localization
Author(s) -
Schauer-Vukasinovic Vesna,
Bur Daniel,
Kling Dorothee,
Grüninger Fiona,
Giller Thomas
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01458-1
Subject(s) - recombinant dna , western blot , immunohistochemistry , microbiology and biotechnology , antibody , epitope , blot , monoclonal antibody , biology , immunofluorescence , chemistry , biochemistry , immunology , gene
A novel aspartic proteinase, called napsin, has recently been found in human and mouse. Due to high similarity with cathepsin D a structural model of human napsin A could be built. Based on this model a potential epitope SFYLNRDPEEPDGGE has been identified, which was used to immunize rabbits. The resulting antibody was employed in monitoring the expression of recombinant human napsin A in HEK293 cell line. Western blot analysis confirmed the specificity of the antibody and showed that human napsin A is expressed as a single chain protein with the molecular weight of approximately 38 kDa. Immunohistochemical studies revealed high expression levels of napsin A in human kidney and lung but low expression in spleen.