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Adenovirus E1A blocks oxidant‐dependent ferritin induction and sensitizes cells to pro‐oxidant cytotoxicity
Author(s) -
Orino Kouichi,
Tsuji Yoshiaki,
Torti Frank M.,
Torti Suzy V.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01443-x
Subject(s) - ferritin , oxidative stress , reactive oxygen species , intracellular , cytotoxic t cell , cytotoxicity , chemistry , transcription factor , microbiology and biotechnology , biochemistry , biology , gene , in vitro
Ferritin is a protein that oxidizes and sequesters intracellular iron in a mineral core. We have reported that the E1A oncogene selectively represses ferritin H transcription, resulting in reduced levels of the ferritin H protein. Here we demonstrate that cells respond to pro‐oxidant challenge by inducing ferritin mRNA and protein, and that this response is completely blocked by E1A. Concordantly, E1A sensitized cells to the cytotoxic effects of oxidative stress and enhanced the accumulation of reactive oxygen species in response to pro‐oxidant challenge. These results demonstrate that expression of E1A impedes the cellular response to oxidative stress, including the induction of ferritin.