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Transcriptional activation of mouse cytosolic chaperonin CCT subunit genes by heat shock factors HSF1 and HSF2
Author(s) -
Kubota Hiroshi,
Matsumoto Shuji,
Yokota Shin-ichi,
Yanagi Hideki,
Yura Takashi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01437-4
Subject(s) - hsf1 , chaperonin , heat shock protein , heat shock factor , protein subunit , microbiology and biotechnology , biology , gene , hspa4 , hspa12a , chaperone (clinical) , heat shock , hspa14 , cytosol , hsp70 , genetics , biochemistry , medicine , pathology , enzyme
The chaperonin containing TCP‐1 (CCT) is a eukaryotic molecular chaperone consisting of eight subunit species and assists in the folding of cytosolic proteins. We show here that all eight mouse CCT subunit genes contain sequences called heat shock elements for binding heat shock transcription factors (HSFs) by electrophoretic mobility shift assays and that these genes are transcriptionally activated by HSFs in reporter gene assays using HeLa cells transiently overexpressing HSFs. These results suggest that HSF1 and/or HSF2 play a role in Cct gene expression.