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Identification of oxidative stress‐regulated genes in rat aortic smooth muscle cells by suppression subtractive hybridization
Author(s) -
Sakamoto Ken'ya,
Yamasaki Yoshimitsu,
Kaneto Hideaki,
Fujitani Yoshio,
Matsuoka Taka-aki,
Yoshioka Rieko,
Tagawa Tomoko,
Matsuhisa Munehide,
Kajimoto Yoshitaka,
Hori Masatsugu
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01419-2
Subject(s) - suppression subtractive hybridization , neointima , vascular smooth muscle , in situ hybridization , biology , microbiology and biotechnology , oxidative stress , gene , gene expression , reactive oxygen species , immunohistochemistry , coactivator , medicine , endocrinology , biochemistry , smooth muscle , immunology , transcription factor , restenosis , cdna library , stent
A suppression subtractive hybridization technique was used to identify reactive oxygen species (ROS)‐regulated genes in rat vascular smooth muscle cells. Three genes out of 89 clones, identified as fibronectin, p105 coactivator and ECA39, showed increased expression after treatment with H 2 O 2 . The mRNA expressions of these three genes were induced in a time‐ and dose‐dependent manner, independent of protein kinase C activation. Immunohistochemical staining showed that the p105 coactivator expression was markedly induced in the neointima of balloon‐injured rat carotid arteries. These results suggest that ROS may play an important role in the development of atherosclerosis by regulating the gene expressions we identified in this study.