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Ascorbate‐mediated electron transfer in protein thiol oxidation in the endoplasmic reticulum
Author(s) -
Csala Miklós,
Braun László,
Mile Valéria,
Kardon Tamás,
Szarka András,
Kupcsulik Péter,
Mandl József,
Bánhegyi Gábor
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01412-x
Subject(s) - chemistry , endoplasmic reticulum , biochemistry , microsome , oxidase test , mitochondrion , ascorbic acid , thiol , electron transfer , enzyme , photochemistry , food science
Addition of, or gulonolactone oxidase‐dependent in situ generation of, ascorbate provoked the oxidation of protein thiols, which was accompanied by ascorbate consumption in liver microsomal vesicles. The maximal rate of protein thiol oxidation was similar upon gulonolactone, ascorbate or dehydroascorbate addition. Cytochrome P450 inhibitors (econazole, proadifen, quercetin) decreased ascorbate consumption and the gulonolactone or ascorbate‐stimulated thiol oxidation. The results demonstrate that the ascorbate/dehydroascorbate redox couple plays an important role in electron transfer from protein thiols to oxygen in the hepatic endoplasmic reticulum, even in gulonolactone oxidase deficient species.