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The outer envelope protein OEP24 from pea chloroplasts can functionally replace the mitochondrial VDAC in yeast
Author(s) -
Röhl Thomas,
Motzkus Michael,
Soll Jürgen
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01399-x
Subject(s) - voltage dependent anion channel , porin , bacterial outer membrane , yeast , mitochondrion , biology , heterologous , chloroplast , saccharomyces cerevisiae , microbiology and biotechnology , biochemistry , chemistry , gene , escherichia coli
The chloroplastic outer envelope protein OEP24 from pea forms a high‐conductance low specificity solute channel as shown by in vitro studies. In order to establish its function also in an in vivo‐like system, the gene encoding OEP24 was transformed into a yeast strain which lacks the general mitochondria solute channel porin, also known as voltage‐dependent anion channel (VDAC). Transformation of the yeast VDAC(−) strain with the OEP24 gene resulted in the recovery of a phenotype indistinguishable from the wild‐type. The OEP24 polypeptide is targeted to the mitochondrial outer membrane in this heterologous system. We conclude that OEP24 forms a solute channel in pea chloroplasts in planta.