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A Synechococcus leopoliensis SAUG 1402‐1 operon harboring the 1‐deoxyxylulose 5‐phosphate synthase gene and two additional open reading frames is functionally involved in the dimethylallyl diphosphate synthesis
Author(s) -
Miller Barbara,
Heuser Thomas,
Zimmer Wolfgang
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01397-6
Subject(s) - operon , biology , open reading frame , gene , atp synthase , genomic dna , biosynthesis , biochemistry , cosmid , genome , escherichia coli , genetics , microbiology and biotechnology , peptide sequence
Experiments have been performed to prove the existence and the functionality of the novel mevalonate independent 1‐deoxyxylulose 5‐phosphate isoprenoid biosynthesis pathway in cyanobacteria. For this purpose, a segment of the 1‐deoxyxylulose 5‐phosphate synthase gene ( dxs ) was amplified from Synechococcus leopoliensis SAUG 1402‐1 DNA via PCR using oligonucleotides for conserved regions of dxs . Subsequent hybridization screening of a genomic cosmid library of S. leopoliensis with this segment has led to the identification of an 18.7 kbp segment of the S. leopoliensis genome on which a dxs homologous gene and two adjacent open reading frames organized in one operon could be localized by DNA sequencing. The three genes of the operon were separately expressed in Escherichia coli , proving that the identified cyanobacterial dxs is functionally involved in the formation of dimethylallyl diphosphate, one basic intermediate of isoprenoid biosynthesis.