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Chloroplast ribonucleoproteins are associated with both mRNAs and intron‐containing precursor tRNAs
Author(s) -
Nakamura Takahiro,
Ohta Masaru,
Sugiura Masahiro,
Sugita Mamoru
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01390-3
Subject(s) - rna splicing , ribonucleoprotein , chloroplast , intron , immunoprecipitation , rna , biology , microbiology and biotechnology , nucleic acid , transfer rna , messenger rna , gene , biochemistry
Tobacco chloroplasts possess five conserved ribonucleoproteins (cpRNPs). To elucidate the function of cpRNPs we analyzed their localization and target nucleic acid molecules in chloroplasts. Immunoprecipitation of the stromal extract and Northern analysis revealed that cpRNPs are associated in vivo with not only various species of chloroplast mRNAs but also intron‐containing precursor (pre‐) tRNAs. This observation strongly suggests that cpRNPs are involved in RNA processing, including mRNA stability and pre‐tRNA splicing.