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Catalytic subunit of telomerase expression is related to RNA component expression
Author(s) -
Stanta Giorgio,
Bonin Serena,
Niccolini Benedetta,
Raccanelli Anna,
Baralle Francisco
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01357-5
Subject(s) - telomerase , ribonucleoprotein , telomere , protein subunit , rna , microbiology and biotechnology , biology , messenger rna , telomerase rna component , telomerase reverse transcriptase , gene expression , microdissection , gene , biochemistry
Telomere length is maintained by the ribonucleoprotein enzyme telomerase. The RNA component of telomerase (hTR) is widespread, and only the expression of the mRNA encoding the catalytic protein subunit (hTRT) is correlated with telomerase activity. We have studied the level of expression of hTR and hTRT in four different models of neoplastic and preneoplastic lesions using the RT‐PCR method on RNA extracted from paraffin‐embedded human tissues after microdissection. The expression at the mRNA level was compared with the enzymatic activity. Our results suggest that there may be a reciprocal control at the transcriptional level of the expression of hTRT and hTR which in turn is associated with tumor progression.