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The possible mechanism of synergistic effects of ethanol, zinc and insulin on DNA synthesis in NIH 3T3 fibroblasts
Author(s) -
She Qing-Bai,
Huang Jin-Sheng,
Mukherjee Jagat J,
Crilly Karan S,
Kiss Zoltan
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01349-6
Subject(s) - insulin , zinc , dna synthesis , kinase , insulin receptor , chemistry , cyclin , fibroblast , 3t3 cells , microbiology and biotechnology , biochemistry , biology , cell cycle , dna , endocrinology , cell , in vitro , insulin resistance , gene , transfection , organic chemistry
In serum‐starved NIH 3T3 fibroblast cultures, zinc (15–40 μM) enhanced both the individual and combined stimulatory effects of insulin and ethanol (EtOH) on DNA synthesis. Zinc, but not EtOH, also promoted the stimulatory effects of insulin on activating phosphorylation of p42/p44 mitogen‐activated protein (MAP) kinases. In the presence of zinc, insulin induced premature expression of cyclin E during early G1 phase; EtOH partially restored the normal timing (late G1 phase) of cyclin E expression. The results suggest that zinc and EtOH promote insulin‐induced DNA synthesis by different mechanisms; while zinc acts by enhancing the effects of insulin on MAP kinase activation, EtOH may act by ensuring timely zinc‐dependent insulin‐induced expression of cyclin E.