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Cloning of mouse mitochondrial thymidine kinase 2 cDNA
Author(s) -
Wettin Katarina,
Johansson Magnus,
Zheng Xinyu,
Zhu Chaoyong,
Karlsson Anna
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01325-3
Subject(s) - thymidine kinase , complementary dna , cloning (programming) , microbiology and biotechnology , mitochondrial dna , mitochondrion , thymidine , biology , nucleoside , nucleoside diphosphate kinase , fusion protein , biochemistry , chemistry , kinase , gene , dna , virology , recombinant dna , virus , herpes simplex virus , computer science , programming language
Phosphorylation of anti‐viral nucleoside analogs by mitochondrial thymidine kinase 2 (TK2) has been implicated as a mechanism for the mitochondrial toxicity caused by several of these compounds. We have cloned the cDNA of mouse TK2 and shown that the enzyme is targeted to the mitochondria when expressed in fusion with the green fluorescent protein. This is the first report on the cloning of a mitochondrial TK2 and will contribute to elucidate the role of TK2 in the pharmacological activation of nucleoside analogs.