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Identification of residues involved in binding of IL5 to β com using β IL3 and β com chimeras
Author(s) -
Czabotar Peter E,
Holland John,
Sanderson Colin J
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01323-x
Subject(s) - receptor , alpha chain , biology , microbiology and biotechnology , stereochemistry , biochemistry , chemistry
In mice there are two forms of the beta chain used in the IL3 receptor system, β com and β IL3 . β com is used by the IL3, IL5 and GM‐CSF receptors whereas β IL3 is only used in the IL3 receptor. In this work an assay was developed to identify residues of β IL3 that restrict IL5 activity. It was found that such residues reside within the 2nd CRM of the molecule. Furthermore, when residues in the β IL3 B′‐C′ loop were replaced with β com sequence a form of β IL3 was produced that was able to respond to IL5. This region is also responsible for IL3 binding to β IL3 in the absence of alpha chain. It is therefore an important structural motif of β com and β IL3 responsible for both ligand interaction and specificity.