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Cloning and characterization of a rabbit ortholog of human Gα16 and mouse Gα15
Author(s) -
Feild John A.,
Foley James J.,
Testa Tania T.,
Nuthulaganti Parvathi,
Ellis Catherine,
Sarau Henry M.,
Ames Robert S.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01317-4
Subject(s) - complementary dna , microbiology and biotechnology , biology , g alpha subunit , cdna library , interleukin 10 receptor, alpha subunit , transfection , protein subunit , peptide sequence , interleukin 5 receptor alpha subunit , hek 293 cells , cloning (programming) , alpha (finance) , gi alpha subunit , receptor , g protein , gene , biochemistry , medicine , construct validity , nursing , computer science , patient satisfaction , programming language
A cDNA was cloned from a rabbit spleen cDNA library which encoded a G‐protein α subunit peptide of 374 amino acids, that at the peptide level exhibited 86% and 79% identity with human Gα16 and mouse Gα15, respectively. The rabbit Gα subunit cDNA was subcloned into a mammalian expression vector and transiently co‐transfected into HEK‐293 cells along with cDNAs encoding the human C3a, C5a, or nociceptin/orphanin FQ receptors. In all three cases the rabbit G α subunit behaved similarly to Gα15 or Gα16 and effectively coupled the transfected receptors to intracellular calcium mobilization pathways. By nucleotide sequence homology and functional activity the rabbit Gα subunit appears to be the ortholog of human Gα16 and mouse Gα15.