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Identification of proteins that interact with NF‐YA
Author(s) -
Yamada Kazuya,
Osawa Haruhiko,
Granner Daryl K.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01311-3
Subject(s) - homeobox , amino acid , serine , protein subunit , glutamine , transcription factor , zinc finger , in vitro , two hybrid screening , identification (biology) , biochemistry , biology , chemistry , yeast , microbiology and biotechnology , gene , phosphorylation , ecology
We used the yeast two‐hybrid system to show that the serum response factor (SRF) and zinc‐fingers and homeobox 1 (ZHX1) proteins interact with the A subunit of nuclear factor‐Y (NF‐YA). GST pulldown assays revealed that both proteins interact specifically with NF‐YA in vitro. Amino acids located between 272 and 564, a region that contains two homeodomains, are required for the interaction of ZHX1 with NF‐YA. Two different domains of NF‐YA, a glutamine‐rich region and a serine/threonine‐rich region, are necessary for the interactions with ZHX1 and SRF, respectively.