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Functional complementation of the Schizosaccharomyces pombe wis1 mutant by Arabidopsis MEK1 and non‐catalytic enhancement by CTR1
Author(s) -
Pan Zaiqun,
Chang Caren
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01306-x
Subject(s) - schizosaccharomyces pombe , arabidopsis , complementation , mutant , map kinase kinase kinase , microbiology and biotechnology , kinase , mapk/erk pathway , mitogen activated protein kinase kinase , biology , protein kinase a , function (biology) , arabidopsis thaliana , schizosaccharomyces , gene , genetics
Arabidopsis thaliana MEK1 encodes a MAPKK homolog whose role in plants is currently unknown. High (but not low) expression of MEK1 rescued the Δ wis1 (MAPKK) mutant of the Schizosaccharomyces pombe Win1/Wis4‐Wis1‐Sty1 stress‐activated MAPK pathway. Rescue was dependent upon upstream and downstream components of the pathway, suggesting that MEK1 might function in a homologous MAPK pathway in plants. When MEK1 was expressed at a low level, rescue of Δ wis1 was achieved by co‐expressing Arabidopsis CTR1 (a putative MAPKK kinase (MAPKKK)). CTR1 constructs alone did not rescue the pathway, indicating that CTR1 augmented MEK1 function. Further data indicated that this enhancement was not due to CTR1 kinase activity.