Premium
Transforming growth factor‐β 1 inhibits expression of selenoprotein P in cultured human liver cells
Author(s) -
Mostert Volker,
Dreher Ingeborg,
Köhrle Josef,
Abel Josef
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01298-3
Subject(s) - selenoprotein p , transforming growth factor , cell culture , luciferase , gene expression , microbiology and biotechnology , tumor necrosis factor alpha , messenger rna , transforming growth factor beta , biology , cytokine , selenoprotein , endocrinology , cell growth , medicine , chemistry , gene , transfection , immunology , oxidative stress , glutathione peroxidase , biochemistry , superoxide dismutase , genetics
The effect of cytokines on the expression of selenoprotein P (SeP) in the human liver cell line HepG2 was investigated. Treatment with interleukin‐1β, interferon‐γ, and tumor necrosis factor‐α had no effect on SeP levels in culture media or on SeP mRNA expression. Conversely, Western analysis revealed a dose‐dependent reduction of SeP content in culture medium after treatment with transforming growth factor (TGF)‐β 1 with an IC 50 of 31 pM. Treatment with 100 pM TGF‐β 1 for 48 h led to a decrease to 21±9% of controls. RT‐PCR analysis of SeP mRNA expression demonstrated an inhibition of SeP transcription to 40±2% of control levels after 24 h. The expression of a luciferase reporter construct under control of the human SeP promoter was downregulated by TGF‐β 1 treatment in a dose‐dependent fashion indicating a transcriptional regulation of the SeP gene by TGF‐β 1 .