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Isolation and characterization of the E2F‐like gene in plants
Author(s) -
Sekine Masami,
Ito Masaki,
Uemukai Kenji,
Maeda Yoshio,
Nakagami Hirofumi,
Shinmyo Atsuhiko
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01296-x
Subject(s) - biology , e2f , cauliflower mosaic virus , microbiology and biotechnology , nicotiana tabacum , gene , reporter gene , transcription factor , dna binding domain , taf2 , tobacco mosaic virus , promoter , gene expression , genetics , virus , transgene , genetically modified crops
The transcription factor E2F regulates the expression of genes involved in the progression of G1/S transition and DNA replication in mammalian cells. We cloned and characterized a cDNA ( NtE2F ) corresponding to a E2F homolog of tobacco ( Nicotiana tabacum ). The transcription of NtE2F was induced as cells progressed from G1 to the S phase and expressed much earlier than that of the proliferating cell nuclear antigen (PCNA) gene. We demonstrated that NtE2F can interact with the tobacco retinoblastoma (Rb)‐related protein in a yeast two‐hybrid assay. To further characterize NtE2F, the trans ‐activation activity of NtE2F was examined by using a transient assay in the tobacco Bright Yellow‐2 (BY‐2) cells with NtE2F fused to the DNA‐binding domain of the yeast transcriptional activator GAL4. NtE2F activated the transcription of the β‐glucuronidase (GUS) reporter gene driven by a cauliflower mosaic virus (CaMV) 35S core promoter containing the GAL4‐binding sequence. This is the first report of the identification of a functionally equivalent E2F‐like gene in plants.