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The regiochemical distribution of positive charges along cholesterol polyamine carbamates plays significant roles in modulating DNA binding affinity and lipofection
Author(s) -
Geall Andrew J,
Eaton Michael A.W,
Baker Terry,
Catterall Catherine,
Blagbrough Ian S
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01262-4
Subject(s) - ethidium bromide , dna , polyamine , chemistry , spermine , dna condensation , transfection , biochemistry , liposome , biophysics , recombinant dna , microbiology and biotechnology , gene , biology , enzyme
We have quantified the effects of the regiochemical distribution of positive charges along the polyamine moiety in lipopolyamines for DNA molecular recognition. High affinity binding leads to charge neutralisation, DNA condensation and ultimately to lipofection. Binding affinities for calf thymus DNA were determined using an ethidium bromide displacement assay and condensation was detected by changes in turbidity using light scattering. The in vitro transfection competence of cholesterol polyamine carbamates was measured in CHO cells. In the design of DNA condensing and transfecting agents for non‐viral gene therapy, the interrelationship of ammonium ions, not just their number, must be considered.