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High‐resolution immunogold cytochemistry indicates that AQP4 is concentrated along the basal membrane of parietal cell in rat stomach
Author(s) -
Fujita Akikazu,
Horio Yoshiyuki,
Nielsen Søren,
Nagelhus Erlend A,
Hata Fumiaki,
Ottersen Ole Petter,
Kurachi Yoshihisa
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01256-9
Subject(s) - immunogold labelling , parietal cell , basement membrane , cytochemistry , foveolar cell , immunocytochemistry , gastric chief cell , biology , enterochromaffin like cell , stomach , gastric glands , gastric mucosa , basal (medicine) , microbiology and biotechnology , chemistry , biophysics , ultrastructure , anatomy , biochemistry , endocrinology , insulin
Gastric parietal cells secrete hydrochloric acid in stomach. Because the secreted HCl solution is isotonic with the plasma fluid, it should accompany the water transport across the membranes of parietal cells. Aquaporins (AQPs) are water channel proteins that play the central role in the cellular handling of water in various mammalian tissues. Using immunocytochemistry, we found that AQP4 was expressed only in parietal cells of rat gastric mucosa. Immunogold electron microscopy study further demonstrated that AQP4 was mostly localized at the basal membrane of parietal cells. In the basal membrane, AQP4 was prominently enriched on the portion contacting with the basement membrane surrounding gastric glands. These results suggest that the contact between basement membrane and basal membrane may generate the signal involved in the targeting of AQP4 in gastric parietal cells.