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Effects of CB 1 cannabinoid receptor activation on cerebellar granule cell nitric oxide synthase activity
Author(s) -
Hillard Cecilia J,
Muthian Shanmugam,
Kearn Christopher S
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01253-3
Subject(s) - chemistry , cannabinoid receptor , pertussis toxin , agonist , nitric oxide synthase , cannabinoid , inverse agonist , receptor , nitric oxide , pharmacology , medicine , endocrinology , biophysics , biochemistry , biology , g protein , organic chemistry
Cerebellar granule cells (CGCs) express the CB 1 subtype of cannabinoid receptor. CB 1 receptor agonists Win 55212‐2, CP55940 and HU210 inhibit KCl‐induced activation of nitric oxide synthase (NOS) in CGCs. Win 55212‐2 has no effect on either basal NOS activity or on activation by N ‐methyl‐ D ‐aspartate and its effect is abolished by pre‐treatment of the cells with pertussis toxin. The CB 1 receptor antagonist/inverse agonist SR141716A both reverses the effects of Win 55212‐2 and produces an increase in NOS activity that is additive with KCl. These results support the hypothesis that activation of the CB 1 receptor in CGCs results in a decreased influx of calcium in response to membrane depolarization, resulting in a decreased activation of neuronal NOS.