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Identification of the cDNA encoding human 6‐phosphogluconolactonase, the enzyme catalyzing the second step of the pentose phosphate pathway
Author(s) -
Collard François,
Collet Jean-François,
Gerin Isabelle,
Veiga-da-Cunha Maria,
Van Schaftingen Emile
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01247-8
Subject(s) - pentose phosphate pathway , biochemistry , dehydrogenase , hexose , complementary dna , biology , escherichia coli , phosphogluconate dehydrogenase , isomerase , glyceraldehyde 3 phosphate dehydrogenase , enzyme , gene , microbiology and biotechnology , glucose 6 phosphate dehydrogenase , glycolysis
We report the sequence of a human cDNA encoding a protein homologous to devB (a bacterial gene often found in proximity to the gene encoding glucose‐6‐phosphate dehydrogenase in bacterial genomes) and to the C‐terminal part of human hexose‐6‐phosphate dehydrogenase. The protein was expressed in Escherichia coli , purified and shown to be 6‐phosphogluconolactonase, the enzyme catalyzing the second step of the pentose phosphate pathway. Sequence analysis indicates that bacterial devB proteins, the C‐terminal part of hexose‐6‐phosphate dehydrogenase and yeast Sol1–4 proteins are most likely also 6‐phosphogluconolactonases and that these proteins are related to glucosamine‐6‐phosphate isomerases.