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Production of fungal fructosyl amino acid oxidase useful for diabetic diagnosis in the peroxisome of Candida boidinii
Author(s) -
Sakai Yasuyoshi,
Yoshida Hiroyuki,
Yurimoto Hiroya,
Yoshida Nobuyuki,
Fukuya Hiroshi,
Takabe Keiji,
Kato Nobuo
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01245-4
Subject(s) - peroxisome , biochemistry , oxidase test , enzyme , escherichia coli , strain (injury) , chemistry , d amino acid oxidase , amino acid , mixed function oxidase , biology , gene , anatomy , cytochrome
A high‐level production of fructosyl amino acid oxidase (FAOD), whose production was toxic in Escherichia coli , was investigated through attempts to utilize the peroxisome of Candida boidinii as the place for protein accumulation. The alcohol oxidase‐depleted strain (strain aod1 Δ) produced FAOD at a four to five times higher level than the wild type strain in terms of protein amount and enzyme activity, although the transcriptional level was similar. As a result of this study, we could improve FAOD productivity approximately 47‐fold from the original transformant, and FAOD accumulated within membrane‐bound peroxisomes up to 18% of the total soluble proteins.