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Knockout of the Sendai virus C gene eliminates the viral ability to prevent the interferon‐α/β‐mediated responses
Author(s) -
Gotoh Bin,
Takeuchi Kenji,
Komatsu Takayuki,
Yokoo Junko,
Kimura Yoshinobu,
Kurotani Atsushi,
Kato Atsushi,
Nagai Yoshiyuki
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01241-7
Subject(s) - sendai virus , interferon , virus , virology , biology , gene , gene knockout , recombinant dna , knockout mouse , genetics
Sendai virus (SeV) renders cells unresponsive to interferon (IFN)‐α. To identify viral factors involved in this process, we examined whether recombinant SeVs, which could not express V protein, subsets of C proteins (C, C′, Y1 and Y2) or any of four C proteins, retained the capability of impeding IFN‐α‐mediated responses. Among these viruses, only the 4C knockout virus completely lost the ability to suppress the induction of IFN‐α‐stimulated gene products and the subsequent establishment of an anti‐viral state. These findings reveal crucial roles of the SeV C proteins in blocking IFN‐α‐mediated responses.