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Regulation of mouse somatostatin receptor type 2 gene expression by glucocorticoids
Author(s) -
Kraus Jürgen,
Wöltje Michael,
Höllt Volker
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01236-3
Subject(s) - somatostatin receptor , promoter , reporter gene , somatostatin , somatostatin receptor 3 , somatostatin receptor 2 , glucocorticoid receptor , biology , response element , hormone response element , somatostatin receptor 1 , gene , gene expression , receptor , electrophoretic mobility shift assay , transcription factor , microbiology and biotechnology , endocrinology , glucocorticoid , genetics , cancer , estrogen receptor , breast cancer
Somatostatin is a regulatory peptide with important inhibitory functions. Its effects are mediated by five receptors, sst1–sst5. Previous studies revealed that sst2 contains three alternative, tissue specific promoters. Here, molecular mechanisms for the regulation of sst2 transcription by glucocorticoids were investigated. Reporter gene assays in NG 108‐15 cells and electrophoretic mobility shift assays revealed that a glucocorticoid responsive element at position −1044 on the second promoter mediates dexamethasone induction. These findings, the existence of a cAMP response element and the tissue dependent activity (brain, pituitary and gastrointestinal tissues) indicate the importance of the second sst2 gene promoter.