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Isolation and purification of a 316 Da preformed compound from strawberry ( Fragaria ananassa ) leaves active against plant pathogens
Author(s) -
Filippone M.P,
Diaz Ricci J,
Mamanı́ de Marchese A,
Farı́as R.N,
Castagnaro A
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01231-4
Subject(s) - fragaria , antimicrobial , chemistry , protease , fast protein liquid chromatography , hydrolysis , chromatography , biology , botany , high performance liquid chromatography , enzyme , biochemistry , organic chemistry
An antibiotic called fragarin showing activities against bacterial and fungal plant pathogens was isolated and purified by FPLC chromatography from the soluble fraction of strawberry leaves. The molecular weight value determined by mass spectrometry is 316 Da. Fragarin remains fully active after protease treatment or alkaline hydrolysis at 100°C for 20 min. Biological and chemical analyses suggest that fragarin may be a new type of an antimicrobial preformed compound – phytoanticipin – and would constitute a primary non‐specific barrier of strawberry defense.