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Synergistic effects of nuclear factors – GATA, VBP and ER in potentiating vitellogenin gene transcription
Author(s) -
Teo Bee Yen,
Tan Nguan Soon,
Lam Toong Jin,
Ding Jeak Ling
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01212-0
Subject(s) - vitellogenin , promoter , transcription factor , biology , gene , context (archaeology) , gata transcription factor , microbiology and biotechnology , gene expression , transfection , regulation of gene expression , transcriptional regulation , transcription (linguistics) , genetics , paleontology , linguistics , philosophy
The Oreochromis aureus vitellogenin (OaVtg) gene contains three imperfect oestrogen response elements (EREs) and GATA and VBP (vitellogenin binding protein) binding sites. An analysis of the promoter indicates that the 5′‐flanking region up to position −625 is sufficient to mediate E 2 control. Furthermore, transfection of deletion and mutagenised promoters indicates that both GATA and VBP synergise with ER, and thus contribute to the regulation of the endogenous OaVtg gene. These findings support the notion that the interplay of promoter elements mediates proper hormone‐dependent and tissue‐specific expression of the OaVtg gene, regardless of non‐consensus sequence context of EREs and VBP.