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Molecular cloning of EDG‐3 and N‐Shc genes from the puffer fish, Fugu rubripes , and conservation of synteny with the human genome
Author(s) -
Yamaguchi Fuminori,
Yamaguchi Kumiko,
Tokuda Masaaki,
Brenner Sydney
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01210-7
Subject(s) - fugu , synteny , biology , gene , exon , genetics , human genome , genome , homology (biology) , intron
EDG‐3 is a receptor for sphingosine‐1‐phosphate mapped on human chromosome 9q22.1–q22.2. We used the compact Fugu genome for its linkage analysis. The Fugu EDG‐3 was composed of one intron and two exons, encoding a 384 amino acid protein that has 56.9% homology with the human EDG‐3. Approximately 3 kb apart, a neuronal Shc (N‐Shc) gene was identified. It spans 7 kb containing 12 coding exons, and has an overall 53.4% similarity with the human protein. We mapped the human N‐Shc gene to chromosome 9q21.3–q22.2. This is the first report of the genomic structure and the linkage of these two genes conserved between Fugu and human.