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Hepatoprotective action of adenovirus‐transferred HNF‐3γ gene in acute liver injury caused by CCl 4
Author(s) -
Nakamura Takafumi,
Akiyoshi Hideo,
Shiota Goshi,
Isono Masato,
Nakamura Kyoko,
Moriyama Masatsugu,
Sato Kenzo
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01202-8
Subject(s) - carbon tetrachloride , hepatocyte nuclear factors , hepatocyte , liver injury , ccl4 , transcription factor , hepatocyte nuclear factor 4 , gene , recombinant dna , medicine , hepatocyte growth factor , endocrinology , biology , microbiology and biotechnology , chemistry , biochemistry , in vitro , receptor , organic chemistry , nuclear receptor
Hepatocyte nuclear factor‐3γ (HNF‐3γ) is an important regulator of liver‐specific genes and the expression of this factor is reduced in the liver injured by carbon tetrachloride (CCl 4 ). Wistar rats were infected with a recombinant adenovirus carrying the cDNA for HNF‐3γ (AxCAHNF3γ) via the tail vein and were treated with CCl 4 by intraperitoneal injection. Liver damage, such as swelling of the hepatocytes and increases in serum marker enzymes were markedly alleviated by AxCAHNF3γ infection. Interestingly, hepatocyte growth factor (HGF) was strongly induced in the AxCAHNF3γ‐infected liver. Likewise, HNF‐1α and HNF‐1β levels were increased, but HNF‐3α and HNF‐3β levels were depressed in the liver. Our results suggest that the transduced HNF‐3γ gene leads to a hepatoprotective effect via the induction of HGF by the combined actions of liver‐enriched transcription factors.