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A disulfide conjugate between anti‐tetanus antibodies and HIV (37–72)Tat neutralizes tetanus toxin inside chromaffin cells
Author(s) -
Stein Sylvia,
Weiss Aryeh,
Adermann Knut,
Lazarovici Philip,
Hochman Jacob,
Wellhöner Hans
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01186-2
Subject(s) - conjugate , toxin , intracellular , chemistry , thioether , tetanus , cholera toxin , antibody , disulfide bond , biochemistry , microbiology and biotechnology , biology , stereochemistry , virology , immunology , mathematical analysis , mathematics , vaccination
Conjugates between anti‐tetanus F(ab′) 2 fragments and the (37–72) fragment of the HIV Tat protein were taken up by chromaffin cells, NG108‐15 neurohybridoma cells and Rev‐2‐T‐6 lymphoma cells. The uptake could not be inhibited by competition with (37–72)Tat, but was reduced in the presence of metabolic inhibitors or at low temperature. The disulfide as well as the thioether conjugate were translocated to the cytoplasmic space, but only the disulfide conjugate moderately restored the stimulated transmitter release inhibited by tetanus toxin. Therefore, disulfide conjugates are more promising than thioethers for the neutralization of intracellular antigens. These conjugates provide new tools to study neuroprotection against bacterial neurotoxins.