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Targeted gene delivery into α 9 β 1 ‐integrin‐displaying cells by a synthetic peptide
Author(s) -
Schneider Holm,
Harbottle Richard P.,
Yokosaki Yasuyuki,
Jost Philipp,
Coutelle Charles
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01181-3
Subject(s) - integrin , genetic enhancement , gene delivery , peptide , biology , gene , microbiology and biotechnology , chemistry , cancer research , biochemistry , cell
We have investigated the usefulness of two small synthetic peptides comprising either a linear or a cyclic PLAEIDGIEL domain and a DNA‐binding moiety of 16 lysine residues to mediate gene transfer selectively into α 9 β 1 ‐integrin‐displaying cells. Such specific gene delivery could only be achieved with the peptide containing the cyclic PLAEIDGIEL domain. However, inclusion of the cationic liposome LipofectAMINE into the peptide/DNA complexes resulted for both peptides in efficient gene transfer with significant targeting specificity. Naturally, the integrin α 9 β 1 is present only in a few highly specialised tissues and abundant throughout the human airway epithelia in vivo. Targeting gene vectors to this integrin therefore appears a useful approach to gene therapy of lung diseases such as cystic fibrosis. As the integrin α 9 β 1 is associated with tissue differentiation during foetal development and may cause resurgence of the foetal phenotype in colon cancers, such vectors may also be applicable for prenatal and cancer gene therapy.