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Accumulation of mature mRNA in the nuclear fraction of mammalian cells
Author(s) -
Gondran Pierre,
Amiot Franck,
Weil Dominique,
Dautry François
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01175-8
Subject(s) - chemistry , microbiology and biotechnology , messenger rna , fraction (chemistry) , biophysics , biology , biochemistry , chromatography , gene
Little is known about the nuclear mRNA content of mammalian cells. In this study, we analyzed by Northern blotting with a panel of probes the nuclear and cytoplasmic fractions derived from several rodent cell lines. For most of the genes under study, mature mRNAs could easily be detected in the nuclear fraction and accumulated to higher levels than the corresponding precursors. In addition, significant differences in the nucleo‐cytoplasmic partition of mature mRNAs were observed between genes as well as between cell types (NIH 3T3, CTLL‐2, D3‐ES, PC‐12), indicating that this nuclear accumulation of mRNA is regulated. Thus, while it is usually considered that splicing is the limiting step of pre‐mRNA processing, these results point towards transport or nuclear retention of mRNA as a key determinant of nuclear mRNA metabolism.