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Tissue‐specific alternative splicing of rat brain fructose 6‐phosphate 2‐kinase/fructose 2,6‐bisphosphatase
Author(s) -
Watanabe Fusao,
Furuya Eisuke
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01174-6
Subject(s) - splice , alternative splicing , exon , gene isoform , biology , rna splicing , skeletal muscle , fructose , gene , biochemistry , microbiology and biotechnology , rna , endocrinology
We have reported the occurrence of eight splice variants of rat brain fructose 6‐phosphate 2‐kinase/fructose 2,6‐bisphosphatase (RB2K). In the present study, we quantified these splice variants in various tissues using a RNAse protection assay and found a tissue‐specific pattern of alternative splicing of the RB2K gene. Splice variants containing exon F were specifically expressed in brain. Moreover, exons D and E were spliced in brain, skeletal muscle and heart. Consequently, eight, six, four and two splice variants were expressed in brain, skeletal muscle, heart and liver plus testis, respectively. These results suggest that distinct RB2K isoforms could be involved in regulation of glycolysis in a tissue‐specific manner.