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Tissue‐inherent fate of GPI revealed by GPI‐anchored GFP transgenesis
Author(s) -
Kondoh Gen,
Gao Xing-Hua,
Nakano Yuka,
Koike Hiroko,
Yamada Shuichi,
Okabe Masaru,
Takeda Junji
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01172-2
Subject(s) - green fluorescent protein , transgene , transgenesis , fusion protein , microbiology and biotechnology , biology , genetically modified mouse , recombinant dna , gene , biochemistry , embryo , reproductive biology , embryogenesis
To clarify the fate of glycosylphosphatidylinositol (GPI) in mammals, we developed GPI‐anchored enhanced green fluorescent protein (EGFP‐GPI) and transgenic mice carrying this fusion construct. When it was introduced to culture cells, the EGFP‐GPI protein was correctly sorted to plasma membranes and microsomes depending on GPI biosynthesis. Transgenic mice carrying EGFP‐GPI were found to show a broad transgene expression. Histologically, a prominent polarized localization of EGFP‐GPI protein was observed in various epithelia, the nervous system and liver and secreted from some exocrine glands, as well as non‐polarized presence in non‐epithelial tissues, demonstrating a tissue‐inherent manner of GPI sorting.