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An early salicylic acid‐, pathogen‐ and elicitor‐inducible tobacco glucosyltransferase: role in compartmentalization of phenolics and H 2 O 2 metabolism
Author(s) -
Chong Julie,
Baltz Rachel,
Fritig Bernard,
Saindrenan Patrick
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01154-0
Subject(s) - elicitor , scopoletin , phenylpropanoid , extracellular , glucosyltransferase , biochemistry , umbelliferone , chemistry , phytoalexin , ferulic acid , salicylic acid , biosynthesis , enzyme , medicine , coumarin , alternative medicine , organic chemistry , pathology , resveratrol
Treatment of tobacco cell suspension cultures with a fungal elicitor of defense responses resulted in an early accumulation of the phenylpropanoid glucosyltransferase TOGT, along with the rapid synthesis and secretion of scopolin, the glucoside of scopoletin. Elicitor‐triggered extracellular accumulation of the aglycone scopoletin and of free caffeic and ferulic acids could only be revealed in the presence of diphenylene iodonium, an inhibitor of extracellular H 2 O 2 production. Our results strongly support a role for TOGT in the elicitor‐stimulated production of transportable phenylpropanoid glucosides, followed by the release of free antioxidant phenolics into the extracellular medium and subsequent H 2 O 2 scavenging.