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Characterization of testicular mouse glucosamine 6‐phosphate deaminase (GNPDA)
Author(s) -
Montag Markus,
van der Ven Katrin,
Dörbecker Christine,
van der Ven Hans
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01141-2
Subject(s) - acrosome , acrosome reaction , polyclonal antibodies , sperm , hamster , biology , zona pellucida , microbiology and biotechnology , antibody , andrology , oocyte , immunology , embryo , medicine , botany
Mammalian glucosamine 6‐phosphate deaminase (GNPDA) was first detected in hamster spermatozoa. To further elucidate its role, we have cloned mouse GNPDA and produced a polyclonal rabbit anti‐GNPDA antibody. This antibody recognized a 33 kDa protein in soluble extracts from mouse brain, liver, kidney, muscle, ovary, testis and sperm. Immunofluorescent analysis of the localization of GNPDA in male reproductive tissue revealed its presence in spermatids and in spermatozoa. In spermatids, GNPDA localized close to the developing acrosome vesicle and in spermatozoa close to the acrosomal region. Following the induction of the acrosome reaction, GNPDA fluorescence in spermatozoa was either reduced or GNPDA was absent. These data suggest that GNPDA might play a role in the acrosome reaction.