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Determination of carbohydrate‐deficient transferrin separated by lectin affinity chromatography for detecting chronic alcohol abuse
Author(s) -
Yoshikawa Kiyoshi,
Umetsu Kazuo,
Shinzawa Haruhide,
Yuasa Isao,
Maruyama Katsuya,
Ohkura Takashi,
Yamashita Katsuko,
Suzuki Tsuneo
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01137-0
Subject(s) - carbohydrate deficient transferrin , affinity chromatography , lectin , transferrin , chemistry , agglutinin , chromatography , alcohol abuse , biochemistry , medicine , alcohol , enzyme , alcohol consumption , psychiatry
Carbohydrate‐deficient transferrin (CDT) has been established as a valuable biological marker for detecting chronic alcohol abuse. To improve the diagnostic efficiency, we studied new CDT determination procedures involving the use of lectin affinity chromatography with Allomyrina dichotoma agglutinin (allo A) and Trichosanthes japonica agglutinin I (TJA‐I) to isolate the CDT isoforms CDT‐allo A and CDT‐TJA, respectively. These procedures, based on detection of the CDT‐allo A and CDT‐TJA isoforms in sera, showed high sensitivity (100% and 98%, respectively) and high specificity (93% and 85%, respectively). These results demonstrate that the new procedures involving the use of lectin affinity chromatography are more useful for isolating markers in the CDT test than the conventional charge‐based separation method.