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Structure of the human VIPR2 gene for vasoactive intestinal peptide receptor type 2
Author(s) -
Lutz E.M.,
Shen S.,
Mackay M.,
West K.,
Harmar A.J.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01135-7
Subject(s) - exon , vasoactive intestinal peptide , gene , microbiology and biotechnology , signal peptide , intron , start codon , open reading frame , biology , untranslated region , stop codon , genetics , peptide sequence , chemistry , receptor , messenger rna , neuropeptide
The VPAC 2 (vasoactive intestinal peptide (VIP) 2 ) receptor is a seven‐transmembrane spanning G protein‐coupled receptor which responds similarly to VIP and pituitary adenylate cyclase activating polypeptide (PACAP) in stimulating cAMP production. Recently, we reported the localisation of the human VPAC 2 receptor gene (VIPR2) to chromosome 7q36.3 (Mackay, M. et al. (1996) Genomics 37, 345–353). Here, we describe the characterisation of the VIPR2 gene structure and promoter region. The VIPR2 gene is encoded by 13 exons, the initiator codon of the 438 amino acid open reading frame is located in exon 1 and the termination signal and a poly‐adenylation signal sequence are located in exon 13. The 5′ untranslated region extends 187 bp upstream of the initiator codon and is extremely GC‐rich (80%). The poly‐adenylation signal is located 2416 bp downstream of the stop codon. Intron sizes range from 68 bp (intron 11) to 45 kb (intron 4) and the human gene spans 117 kb.