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A 69 bp fragment in the pyrroline‐5‐carboxylate reductase promoter of Arabidopsis thaliana activates minimal CaMV 35S promoter in a tissue‐specific manner
Author(s) -
Hua Xuejun,
van de Cotte Brigitte,
Van Montagu Marc,
Verbruggen Nathalie
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01110-2
Subject(s) - arabidopsis thaliana , biology , gene , arabidopsis , reductase , microbiology and biotechnology , enzyme , mutant , biochemistry
The Arabidopsis thaliana gene that encodes pyrroline‐5‐carboxylate reductase ( At‐P5R ), the last enzyme in proline biosynthesis in A. thaliana , is developmentally regulated and is highly expressed in cells that divide rapidly or undergo changes in osmotic potential. A 69 bp region (P69; −120 to −51) has previously been identified in a 5′ deletion analysis of the At‐P5R promoter to be necessary for the basal expression. Here, the essential role of P69 for tissue‐specific expression of At‐P5R is demonstrated by loss‐ and gain‐of‐function experiments.