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Proteinase 3 mRNA expression is induced in monocytes but not in neutrophils of patients with cystic fibrosis
Author(s) -
Just Jocelyne,
Moog-Lutz Christel,
Houzel-Charavel Anne,
Canteloup Sandrine,
Grimfeld Alain,
Witko-Sarsat Véronique,
Cayre Yvon E.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01098-4
Subject(s) - proteinase 3 , messenger rna , cystic fibrosis , monocyte , downregulation and upregulation , sputum , inflammation , proteinase k , microbiology and biotechnology , s100a9 , chemistry , immunology , biology , medicine , pathology , enzyme , gene , myeloperoxidase , biochemistry , tuberculosis
Proteinase 3 (PR3), a serine proteinase which can degrade lung tissue, is present in the cystic fibrosis (CF) sputum. In the present study, PR3 protein and mRNA expression was determined in circulating neutrophils and monocytes. CF neutrophils contained similar PR3 concentrations as healthy controls and poorly expressed PR3 mRNA. In contrast, CF monocytes showed significantly higher PR3 concentrations than controls, together with an upregulation of PR3 mRNA expression especially during pulmonary exacerbation. Interestingly, antibiotic treatment fully abrogated PR3 mRNA expression and decreased PR3 protein in monocytes. Our findings highlight a potential role of monocyte‐derived PR3 in CF‐associated airway inflammation.