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Distinct roles of profilin in cell morphological changes: microspikes, membrane ruffles, stress fibers, and cytokinesis
Author(s) -
Suetsugu Shiro,
Miki Hiroaki,
Takenawa Tadaomi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01086-8
Subject(s) - profilin , cytokinesis , microbiology and biotechnology , actin , actin remodeling , cdc42 , cell division , mdia1 , actin remodeling of neurons , chemistry , biology , microfilament , actin cytoskeleton , cell , cytoskeleton , biochemistry
Here we report the functional importance of profilin in various actin‐mediated morphological changes using H119E mutant profilin I, which is deficient only in actin binding. In the case of actin‐protrusive structures from the plasma membrane, H119E‐profilin was shown to suppress the formation of Cdc42‐induced actin microspikes and Rac‐induced membrane ruffles. Conversely, Rho‐induced stress fiber formation seemed to occur independently of H119E‐profilin introduction. Furthermore, H119E‐profilin blocked cleavage furrow ingression and subsequent adhesion to the substratum during cell division, a process in which actin plays indispensable roles.