Premium
Molecular cloning and characterization of AtTERT, a telomerase reverse transcriptase homolog in Arabidopsis thaliana
Author(s) -
Oguchi Keiko,
Liu Hongtu,
Tamura Katsunori,
Takahashi Hideo
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01083-2
Subject(s) - reverse transcriptase , complementary dna , telomerase , telomerase reverse transcriptase , microbiology and biotechnology , biology , open reading frame , arabidopsis thaliana , rapid amplification of cdna ends , reverse transcription polymerase chain reaction , messenger rna , molecular cloning , rna , genetics , gene , peptide sequence , mutant
On the basis of its predicted homology to human telomerase reverse transcriptase (hTERT), a cDNA for Arabidopsis thaliana TERT (AtTERT) has now been isolated from cultured cells. The cDNA contains an open reading frame of 3372 bp, encoding a protein with a predicted size of 131 kDa and isoelectric point of 9.9. The AtTERT protein contains the conserved reverse transcriptase motifs 1, 2 and A–E as well as the TERT‐specific T motif. Reverse transcription‐polymerase chain reaction analysis and an assay of telomerase activity revealed that both AtTERT mRNA and telomerase activity are abundant in shoot apical meristems but are not detectable in rosette leaves.