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Induction of apoptosis of monocyte‐macrophage lineage cells by 5‐S‐GAD
Author(s) -
Hijikata Mariko,
Matsumoto Hiroko N.,
Kobayashi Ayako,
Nifuji Akira,
Noda Masaki,
Natori Shunji
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01077-7
Subject(s) - osteoclast , apoptosis , multinucleate , monocyte , microbiology and biotechnology , macrophage , hl60 , chemistry , in vitro , cellular differentiation , lineage (genetic) , biology , immunology , biochemistry , gene
We found that 5‐S‐GAD, an insect‐derived antibacterial peptide, inhibited murine osteoclast formation in vitro. We examined the specific time point of the inhibitory action of 5‐S‐GAD on osteoclast formation and found that it mainly suppressed differentiation of osteoclasts in the middle of the culture period. Using HL60 cells that are able to differentiate into multinucleated macrophage‐like cells, we found that 5‐S‐GAD induced apoptosis of HL60 cells by producing H 2 O 2 . Thus, the inhibition of osteoclast formation by 5‐S‐GAD could be, in part, due to apoptosis of the cells of an osteoclast lineage.