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Molecular cloning of newt sex pheromone precursor cDNAs: evidence for the existence of species‐specific forms of pheromones
Author(s) -
Iwata T.,
Umezawa K.,
Toyoda F.,
Takahashi N.,
Matsukawa H.,
Yamamoto K.,
Miura S.,
Hayashi H.,
Kikuyama S.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01062-5
Subject(s) - complementary dna , cdna library , peptide sequence , biology , amino acid , cloning (programming) , sex pheromone , clone (java method) , molecular cloning , microbiology and biotechnology , peptide , biochemistry , gene , genetics , computer science , programming language
Cloning of cDNA encoding a decapeptide pheromone (sodefrin) that attracts conspecific female newts was attempted. A cDNA clone encoding a protein consisting of 189 amino acid residues including a sodefrin sequence was isolated from a Cynops pyrrhogaster abdominal gland cDNA library. Likewise, a cDNA clone encoding a molecule comparable to the sodefrin precursor was obtained from a Cynops ensicauda abdominal gland cDNA library. This clone encoded a precursor protein of 192 amino acid residues, including a sodefrin‐like peptide sequence with substitutions of two amino acid residues. This is the first report of a peptide pheromone precursor in vertebrates.