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Wide distribution of short interspersed elements among eukaryotic genomes
Author(s) -
Borodulina Olga R.,
Kramerov Dmitri A.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01059-5
Subject(s) - polyadenylation , biology , sine , genome , rna polymerase iii , oligonucleotide , polymerase chain reaction , genetics , terminator (solar) , polymerase , dna , microbiology and biotechnology , computational biology , rna , gene , rna dependent rna polymerase , ionosphere , geometry , mathematics , physics , astronomy
Most short interspersed elements (SINEs) in eukaryotic genomes originate from tRNA and have internal promoters for RNA polymerase III. The promoter contains two boxes (A and B) spaced by ∼33 bp. We used oligonucleotide primers specific to these boxes to detect SINEs in the genomic DNA by polymerase chain reaction (PCR). Appropriate DNA fragments were revealed by PCR in 30 out of 35 eukaryotic species suggesting the wide distribution of SINEs. The PCR products were used for hybridization screening of genomic libraries which resulted in identification of four novel SINE families. The application of this approach is illustrated by discovery of a SINE family in the genome of the bat Myotis daubentoni . Members of this SINE family termed VES have an additional B‐like box, a putative polyadenylation signal and RNA polymerase III terminator.