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R‐esp1, a rat homologue of Drosophila Groucho, is differentially expressed after optic nerve crush and mediates NGF‐induced survival of PC12 cells
Author(s) -
Arndt Marco,
Bank Ute,
Frank Karin,
Sabel Bernhard A.,
Ansorge Siegfried,
Lendeckel Uwe
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01052-2
Subject(s) - nerve growth factor , superior colliculus , microbiology and biotechnology , transcription factor , chemistry , neuroscience , gene , biology , receptor , biochemistry
The differential display reverse transcription polymerase chain reaction method was used to detect alterations in gene expression in the superior colliculus after optic nerve crush in adult rats. One of the most prominent changes observed was the selective induction of R‐esp1, a homologue of the Drosophila enhancer of split locus (Groucho). Therefore, we studied the influence of R‐esp1 on nerve growth factor (NGF)‐induced cell survival of PC12 cells. Overexpression of R‐esp1 promotes cell survival even in the absence of NGF and, conversely, it is reduced by antisense‐mediated inhibition of R‐esp1 expression. In conclusion, we propose a novel model in which R‐esp1 protein mediates the NGF‐signaling pathway.