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Association of the rat heterogeneous nuclear RNA‐ribonucleoprotein F with TATA‐binding protein
Author(s) -
Yoshida Tatsushi,
Makino Yasutaka,
Tamura Taka-aki
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01048-0
Subject(s) - rna splicing , heterogeneous ribonucleoprotein particle , ribonucleoprotein , heterogeneous nuclear ribonucleoprotein , tata binding protein , splicing factor , sr protein , rna binding protein , biology , messenger rna , microbiology and biotechnology , precursor mrna , transcription factor , alternative splicing , transcription (linguistics) , rna , chemistry , dna binding protein , genetics , gene , linguistics , philosophy
Heterogeneous nuclear ribonucleoprotein F (hnRNP‐F) has been shown to be a pre‐mRNA splicing factor. Recent studies have uncovered the coordination of synthesis of pre‐mRNA and its processing, including post‐transcriptional modification and splicing. Here, we present evidence for an association between a splicing factor, hnRNP‐F, and TATA‐binding protein (TBP), which is an essential factor needed for transcription initiation. An affinity detection experiment revealed hnRNP‐F in the preparation of TBP‐interacting proteins. HnRNP‐F was associated with TBP in nuclear extracts and was capable of direct binding to TBP in vitro. These results suggest that hnRNP‐F is associated with TBP in the cell. HnRNP‐F was observed in abundance in the thymus, spleen and testis, and its distribution pattern was similar to that of TBP, implying a functional coordination of transcription and splicing. We assume that the splicing machinery is associated with the transcription apparatus as a prerequisite prior to transcriptional elongation.