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Distribution and quantification of immunoreactive orexin A in rat tissues
Author(s) -
Taheri Shahrad,
Mahmoodi Mehdi,
Opacka-Juffry Jolanta,
Ghatei Mohammad A.,
Bloom Stephen R.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01030-3
Subject(s) - orexin a , medicine , hypothalamus , endocrinology , orexin , brainstem , radioimmunoassay , lateral hypothalamus , central nervous system , biology , appetite , chemistry , neuropeptide , receptor
A sensitive and specific radioimmunoassay for orexin A was developed. Orexin A immunoreactivity was found to be confined to the central nervous system (CNS) with the highest concentrations in the hypothalamus, inferior and superior colliculi and brainstem. Within the hypothalamus, the highest levels were found in the lateral and posterior hypothalamus. These regions had a greater orexin A content in females compared to males. The orexin A content of hypothalamic regions did not change with fasting and no difference was noted in hypothalami of rats fed a high fat diet. The hypothalamic orexin A content was not different in obese Zucker rats compared to lean controls. Thus, orexin A has a wide distribution in the CNS, but appetite regulation may not be its main function.