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Low temperature and aging‐promoted expression of PUMP in potato tuber mitochondria
Author(s) -
Nantes Iseli L.,
Fagian Marcia M.,
Catisti Rosana,
Arruda Paulo,
Maia Ivan G.,
Vercesi Anibal E.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01017-0
Subject(s) - polyclonal antibodies , mitochondrion , senescence , thermogenesis , postharvest , chemistry , linoleic acid , ripening , membrane potential , biochemistry , food science , horticulture , biophysics , microbiology and biotechnology , biology , fatty acid , antibody , genetics , adipose tissue
In this communication, we show that the plant uncoupling mitochondrial protein (PUMP) present in potato tuber mitochondria is induced by aging at 28°C and that this induction is strongly stimulated when the potato tubers are stored at low temperature (4°C). PUMP activity was detected by the degree of linoleic acid (LA)‐induced ATP‐sensitive mitochondrial uncoupling measured as a function of the decrease in membrane potential (Δ Ψ ). The PUMP content was evaluated by immunoblot analysis using polyclonal antibodies raised against potato PUMP that specifically detected a 32 kDa band. In agreement with the effect of LA on Δ Ψ , the content of the 32 kDa band increased during storage and was stimulated by low temperature. These results support the proposed role of PUMP in plant thermogenesis and possibly in fruit ripening and senescence.