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Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase
Author(s) -
Richard Peter,
Toivari Mervi H,
Penttilä Merja
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)01016-9
Subject(s) - xylitol , pichia stipitis , saccharomyces cerevisiae , biochemistry , nad+ kinase , dehydrogenase , open reading frame , sorbitol dehydrogenase , chemistry , xylose , enzyme , yeast , gene , fermentation , peptide sequence
The open reading frame YLR070c of Saccharomyces cerevisiae has high sequence similarity to S. cerevisiae sorbitol dehydrogenase and to xylitol dehydrogenase of Pichia stipitis . Overexpression of this open reading frame in S. cerevisiae resulted in xylitol dehydrogenase activity. The enzyme is specific for NADH. The following Michaelis constants were estimated: D ‐xylulose, 1.1 mM; NADH, 240 μM (at pH 7.0); xylitol, 25 mM; NAD, 100 μM (at pH 9.0). Xylitol dehydrogenase activity with the same kinetic properties can also be induced by xylose in wild type S. cerevisiae cells.